rabbit anti galectin3  (Proteintech)

Journal: Dental materials journal

Article Title: Dose effects of beta-tricalcium phosphate nanoparticles on biocompatibility and bone conductive ability of three-dimensional collagen scaffolds.

doi: 10.4012/dmj.2016-295

Figure Lengend Snippet: Fig. 8 Immunohistologic staining of CD68 and gal3 (A, B, and C), P4HB (D, E, and F), ASMA (G, H, and I), RECA-1 (J, K, and L), and peroxidase activity (PA) (M, N, and O) in rat subcutaneous tissue at 10 days for implanted COL (A, D, G, J, and M), TCP5 (B, E, H, K, and N), and TCP25 (C, F, I, L, and O). CD68, P4HB, ASMA and RECA-1 are shown in red, and gal3 and the nucleus of all cells are shown in green. Arrows indicate peroxidase-positive neutrophils. Scale bars respectively represent 100 µm (A–L) and 20 μm (M–O).

Article Snippet: After pretreatment with 0.3% Triton X-100 and normal donkey serum, the sections were incubated overnight with the following primary antibodies, alone or in mixtures as indicated in the data: mouse antiCD68 (1:100 dilution; AbD Serotec, Kidlington, UK), rabbit anti-galectin3 (gal3) (1:300 dilution, sc-20157; Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse anti-prolyl-4-hydroxylase beta (P4HB) (1:1600 dilution, clone6-9H6; Acris Antibodies, San Diego, CA, USA), mouse anti-rat endothelial cell antigen-1 (RECA1) (1:1000 dilution; AbD Serotec), and mouse antialpha-smooth muscle actin (ASMA) (1:1600 dilution, clone 1A4; Sigma-Aldrich, St. Louis, MO, USA).

Techniques: Staining, Activity Assay